Milli-Mark Bcl-2-Alexa Fluor488; c

Analysis Note

ControlJurkat Cells

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

This Milli-Mark Bcl-2 antibody is validated for use in Flow for the detection of the Milli-Mark Bcl-2 protein.

Disclaimer

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc.

General description

Bcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Bcl-2 also regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspases. It appears to Inhibit caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Further understanding in how Bcl-2 functions and interacts with other proteins to promote or inhibit apoptosis may lead to new treatments for cancer, neurological disorders and autoimmune diseases.

Immunogen

KLH-conjugated, synthetic peptide corresponding to human Bcl-2

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified mouse monoclonal IgG2a conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Protein G Purified

Quality

Evaulated by flow cytometery using Jurkat cells.

Specificity

Antibody recognizes Bcl-2.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

26 kDa Calculated